Brain water content was measured by the dry-wet weight method. Mice were sacrificed 24 h post-TBI. After anesthesia, the brains were removed immediately and divided into ipsilateral hemispheres (with injury) and contralateral hemispheres. The ipsilateral hemispheres were weighed immediately to obtain the wet weight and then dried at 80 °C for 72 h to obtain the dry weight. As previously described 20, the percentage of water content was calculated as follows: brain water content = [(wet weight - dry weight)/wet weight] × 100%.

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