Brain water content was measured by the dry-wet weight method. Mice were sacrificed 24 h post-TBI. After anesthesia, the brains were removed immediately and divided into ipsilateral hemispheres (with injury) and contralateral hemispheres. The ipsilateral hemispheres were weighed immediately to obtain the wet weight and then dried at 80 °C for 72 h to obtain the dry weight. As previously described 20, the percentage of water content was calculated as follows: brain water content = [(wet weight - dry weight)/wet weight] × 100%.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.