The human GC cells (MKN-45, AGS, SGC-7901, and MGC-803) and normal GES-1 gastric mucosal cells were maintained in our lab under standard culture conditions 22. MSC-AS1 shRNA (shMSC-AS1-1 and shMSC-AS1-2) and non-targeting RNA (NT shRNA) were obtained from GenePharma (Shanghai, China). The pcDNA3.1-PFKFB3 and pcDNA3.1-MSC-AS1 vectors were constructed by inserting the cDNA products of PFKFB3 and MSC-AS1 into the expression vector pcDNA3.1 (Invitrogen, Carlsbad, CA, USA). The plasmids were delivered into GC cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

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