Animals were anesthetized with isoflurane and were perfused intracardially with cold (4 °C) heparinized 0.9% saline. For Western blot analysis, brain tissues around the injured site were collected. Samples were immediately frozen in liquid nitrogen, then transferred to a -80 °C freezer until use. For frozen and paraffin sections, the brain was steeped in formaldehyde solution after perfusion.

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