To measure whether CENPF was involved in cell proliferation, we performed the MTT assay. A549 cells were seeded into 96-well plates at a density of 5×103/well and cultured for 24 h after transfection. Cell proliferation was assessed using the MTT Cell Growth Assay Kit (Sigma-Aldrich) according to the manufacturer's instructions. The optical density was measured by a microplate reader set at 490 nm. In order to control the experimental data error, the experiments were performed at least three times.

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