2.6. Prognosis values, protein and mRNA expression analysis of hub genes

We validated the prognostic efficacy of mRNAs using online tool - The Kaplan-Meier plotter database (KMplot, https://kmplot.com/).The KMplot is capable to assess the effect of mRNA on survival in 21 cancer types including breast, liver, ovarian and gastric cancer. The Human Protein Atlas (www.proteinatlas.org) is a Swedish-based program started in 2003 with the aim to map all the human proteins in cells, tissues and organs using integration of various omics technologies. Protein expressions of hub genes in normal and HCC tissues were analyzed in The Human Protein Atlas 17, 18. The mRNA expression level in HCC was further verified using Gene Expression Profiling Interactive Analysis (GEPIA). GEPIA is a web server focusing on tumor and normal gene expression profiling based on the data from TCGA and Genotype-Tissue Expression (GTEx) project 19. |LogFC| > 1 and p < 0.05 were set as thresholds for recognition of significant gene expression.

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