2.6. Prognosis values, protein and mRNA expression analysis of hub genes

We validated the prognostic efficacy of mRNAs using online tool - The Kaplan-Meier plotter database (KMplot, https://kmplot.com/).The KMplot is capable to assess the effect of mRNA on survival in 21 cancer types including breast, liver, ovarian and gastric cancer. The Human Protein Atlas (www.proteinatlas.org) is a Swedish-based program started in 2003 with the aim to map all the human proteins in cells, tissues and organs using integration of various omics technologies. Protein expressions of hub genes in normal and HCC tissues were analyzed in The Human Protein Atlas 17, 18. The mRNA expression level in HCC was further verified using Gene Expression Profiling Interactive Analysis (GEPIA). GEPIA is a web server focusing on tumor and normal gene expression profiling based on the data from TCGA and Genotype-Tissue Expression (GTEx) project 19. |LogFC| > 1 and p < 0.05 were set as thresholds for recognition of significant gene expression.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.