The qRT-PCR was performed using the TB Green Premix Ex Taq II (TaKaRa) on the Applied Biosystems StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). Through this experiment, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference gene and the divergent primers for CENPF and GAPDH were obtained from BioSune Corporation (Shanghai, China). Sequences of primers used for qRT-PCR are as follows: GAPDH, forward primer 5′‐TGACTTCAACAGCGA CACCCA-3′, reverse primer 5′-CACCCTGTTGCTGTAGCCAAA-3′. CENPF, forward primer 5′-AGCACGACTCCAGCTACAAGGT-3′, reverse primer 5′-CATCA TGCTTTGGTGTTCTTTCTG-3′. The PCR conditions were set as the followings: 95 °C, 30 s; followed by 40 cycles at 95 °C, 5 s; finally, 60 °C, 30 s for every specific primer. In the end, melting curves were generated to ensure the specificity of PCR. Finally, the relative expression levels of CENPF were calculated using the 2-ΔΔCT method.

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