From December 2019 to January 2020, a total of 13 pairs of fresh LUAD and adjacent non-tumor lung tissues (5cm distant from lung cancer) were obtained during surgical resection. All these 26 lung tissue samples were enrolled after signed informed consent form in Weihai Municipal Hospital of Shandong Province. All patients did not undergo any chemotherapy or radiation therapy prior to operation, and had no other malignancies accompanied. Of these samples, 13 pairs of LUAD tissues and adjacent non‑tumorous tissues were frozen immediately, stored at ‑80 °C and used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. This study was approved by the ethics committee of Weihai Municipal Hospital (2020024).

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