In the current study, we focused on three genes (JAZF1, IGF1, and IGF2BP2) involved in lipid metabolism, which have been shown to affect insulin resistance 5-11, 13-18. The SNP rs864745 in JAZF1 is located in intron 1 28, 29 and rs35767 is located in the IGF1 promoter 30. The three SNPs (rs4376068, rs4402960, and rs6769511) of IGF2BP2 are all located in intron 2 6, 31.

The QIAamp Blood Mini Kit (Qiagen, Hilden, Germany) was used to extract genomic DNA from peripheral lymphocytes. The aforementioned five SNPs in the three genes were genotyped using the MassArray Analyser 4.0 (Agena Bioscience, Inc., San Diego, CA, USA) as previously described 32. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed using a device from Agena Bioscience. SNP genotyping was performed using a SpectroCHIP array, and the raw genotyping data were obtained using TYPER4.0 software (Agena Bioscience).

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