Non-parametric Mann-Whitney test was used to detect differences in continuous variables. The Pearson's correlation test was performed to examine the correlation between ACE2 expression and the infiltration of immune cells and the expression of immune cell markers. The prognostic value of ACE2 was examined by Kaplan-Meier curve, and log-rank test was utilized to demonstrate the significance of the difference in survival curves. The univariate and multivariate Cox regression analyses of ACE2 were conducted. A P-value < 0.05 indicated statistical significance.

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