Specific-pathogen-free (SPF) C57BL/6N male mice (10-week-old) (CLEA Japan, Inc., Meguro-ku, Tokyo Japan), SPF C57BL/6N male mice (2-day-old) (CLEA Japan, Inc.), and SPF KK-Ay/TaJcl male mice (14-week-old) (CLEA Japan, Inc.) were used. They were housed in individual cages in an air-conditional room at 23 ± 1°C under SPF conditions with a 12 h light/12 h dark cycle and free access to drinking water and a pelleted basal diet. At the time of the last experiment, a blood glucose level of 300 mg/dl or more was considered as diabetes onset in the animal. And also C57BL/6N mice were used in the study, the same result was indicated on the other mouse strain (BALB/c and ICR mice) (data not shown). This study was performed in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of Suzuka University of Medical Science (Approval number: 34). All surgeries were performed under pentobarbital anesthesia, and all efforts were implemented to minimize animal suffering.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.