Specific-pathogen-free (SPF) C57BL/6N male mice (10-week-old) (CLEA Japan, Inc., Meguro-ku, Tokyo Japan), SPF C57BL/6N male mice (2-day-old) (CLEA Japan, Inc.), and SPF KK-Ay/TaJcl male mice (14-week-old) (CLEA Japan, Inc.) were used. They were housed in individual cages in an air-conditional room at 23 ± 1°C under SPF conditions with a 12 h light/12 h dark cycle and free access to drinking water and a pelleted basal diet. At the time of the last experiment, a blood glucose level of 300 mg/dl or more was considered as diabetes onset in the animal. And also C57BL/6N mice were used in the study, the same result was indicated on the other mouse strain (BALB/c and ICR mice) (data not shown). This study was performed in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of Suzuka University of Medical Science (Approval number: 34). All surgeries were performed under pentobarbital anesthesia, and all efforts were implemented to minimize animal suffering.

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