Brain tissue preparation and pathological examination

After the behavioral test, the brain tissue of the rats in each group was removed, stored in 4% paraformaldehyde, and fixed at 4°C for 48 hours. We embedded the samples in the paraffin and sliced them into 5 µm-thick sections. After deparaffinization and rehydration, the tissues were stained with hematoxylin/eosin (HE) and Nissl to detect the histopathological changes of hippocampal neurons under light microscope.

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