At the end of the behavior test, we anesthetized the rats and fully exposed their hearts. Then, we centrifuged the whole blood from the right atrium for 20 minutes and refrigerated the upper serum at -80°C. The serum IL-1β, IL-6, and TNF-α levels were measured via a commercial ELISA kit (BD Biosciences Co., San Jose, CA, USA) according to the manufacturer's protocol.

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