BALB/c male nude mice, 4-6 weeks old, were purchased from the Shanghai SLAC Laboratory Animal Co., Ltd. (Shanghai, China). All animal experiments were conducted in Xinhua hospital Shanghai Jiao Tong University. ECA109 cells (Lv-NC, Lv-NETO2) were resuspended at a density of 2×106 in phosphate-buffered saline and inoculated subcutaneously into the right axilla of the nude mice (5 mice/group). Tumor growth was monitored every week. On day 28, the mice were sacrificed, and the tumors were dissected and weighed. Tumor volume was measured using a digital caliper and calculated as 4 π/3 × length/2 × width2 (mm). All animal experiments were approved by the Animal Care Committee of Xinhua hospital Shanghai Jiao Tong University.

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