Approximately 1000 ECA109 Kyse150 cells were plated in 96-well plates and cultured for 24 h. Cell viability was assessed using Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturer's instructions.

500 cells in log-phase were plated into 6-well plates. After a two-week incubation period, colonies were fixed with 4% paraformaldehyde for 30 min and stained with 0.1% crystal violet solution (Sigma, USA) for 15 min. Colonies (>50 cell) were counted under microscopy.

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