Cells were lysed in RIPA buffer (Cell Signaling, USA) and quantified using the BCA protein assay (Beyotime, Shanghai, China). Equal amounts of protein were subjected to electrophoresis on SDS-PAGE and were probed with the following primary antibodies: NETO2, Nrf2, p-Nrf2 (Ser40) purchased from Abcam. Caspase 3, cleaved caspase 3, Bcl-2, p-AKT (Thr308), AKT, p-mTOR, p-ERK1/2 (Thr202/Tyr204), ERK1/2, E-cadherin, N-cadherin, slug, and snail were purchased from Cell Signaling Technology (Danvers, MA, USA). The protein bands were measured using a Gel Doc 2000 (Bio-Rad).

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