Cells were lysed in RIPA buffer (Cell Signaling, USA) and quantified using the BCA protein assay (Beyotime, Shanghai, China). Equal amounts of protein were subjected to electrophoresis on SDS-PAGE and were probed with the following primary antibodies: NETO2, Nrf2, p-Nrf2 (Ser40) purchased from Abcam. Caspase 3, cleaved caspase 3, Bcl-2, p-AKT (Thr308), AKT, p-mTOR, p-ERK1/2 (Thr202/Tyr204), ERK1/2, E-cadherin, N-cadherin, slug, and snail were purchased from Cell Signaling Technology (Danvers, MA, USA). The protein bands were measured using a Gel Doc 2000 (Bio-Rad).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.