All cell lines were digested with 0.25% trypsin and washed with PBS three times. Then cells were resuspended in 100μL PBS and incubated with anti-CD44-APC (1.25 μl/ test) and anti-CD24-PE (5 μl/ test) (Biolegend, San Diego, USA), or with their controls at 4 °C for 30min. After incubation, the cell distribution was measured using a MACSQuantTM Flow Cytometer (Miltenyi Biotec).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.