Formalin-fixed paraffin-embedded sections of PCa tissues and adjacent non-cancerous tissues were obtained from the first hospital of China Medical University. Ethical approval for sample collection and tissue use was approved by the Human Research Ethics Committee of China Medical University. A total of 162 tissues, including 20 PCa tissues without matched adjacent non-cancerous tissues, and 71 pairs of PCa and matched adjacent non-cancerous tissues, were collected to perform IHC staining. IHC experiments were performed as previously described 12. The immunohistochemical stain of PRPF6 expression was evaluated by the Hscore method, as previously described 26. Briefly, the intensity was scored as follows: 0, negative; 1, weak; 2, moderate and 3, strong. The final score, which ranged from 0 to 3, was determined by summing the intensity score multiplied by the corresponding proportion.

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