Cell viability was measured using the AlamarBlue Cell Viability Reagent (Invitrogen, Carlsbard, CA, USA) according to the manufacturer's instructions. In brief, after cells were cultured in medium supplemented with 10% alamarBlue for 2 h, 100 μl of the culture medium was transferred to 96-well plates and quantified spectrophotometrically with a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) at wavelengths of 570 nm and 650 nm. The culture medium from no cell-seeded disks was similarly analyzed as blank controls.

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