Cell viability was measured using the AlamarBlue Cell Viability Reagent (Invitrogen, Carlsbard, CA, USA) according to the manufacturer's instructions. In brief, after cells were cultured in medium supplemented with 10% alamarBlue for 2 h, 100 μl of the culture medium was transferred to 96-well plates and quantified spectrophotometrically with a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) at wavelengths of 570 nm and 650 nm. The culture medium from no cell-seeded disks was similarly analyzed as blank controls.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.