To identify whether IFN-γ, rapamycin or kynurenine treated cancer cells impact the phagocytic activity and polarization of human monocyte / macrophage cell line U937, HeLa and SiHa cells were pretreated with recombinant human IFN-γ protein (rhIFN-γ, 10ng/ml, PeproTech), rapamycin (2μmol/l, Sigma), or kynurenine (500μmol/l, Sigma) for 48 hours, supernatant was discarded and cells were washed with PBS. Then, fresh medium and U937 cells were added to the plate and cervical cancer cells were co-culture directly with U937 cells for 48 hours at the ratio of 1:1. After 48 hours, cells were harvested and analyzed by FCM.

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