Aorta paraffin block slides were de-paraffinized and incubated with 0.3% H2O2 (Sigma-Aldrich) for 30 mins, rinsed 3 times with PBS, incubated in normal animal serum to block antibody binding, incubated with antibodies (listed in Supplementary Table 2) for 2 days at 4°C and then rinsed 3 times with PBS. Slides were then treated with biotinylated secondary antibodies in the ABC kit (Vector Laboratories), incubated for 1 h at room temperature in blocking solution, and rinsed 3 times with PBS. Slides were developed with 3, 3´-diaminobenzidine (DAB) substrate containing 0.3% H2O2 at room temperature for 15 min and mounted with cover slips using DPX mounting solution (Sigma-Aldrich). Stained images were visualized by light microscopy (Olympus Optical Co., Tokyo, Japan) [28].

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