The FeCl3 thrombosis model was modified based on previous reports [30,31]. Briefly, rats were anesthetized by intraperitoneal injection of 1.2 g/kg urethane 24 h after adenoviral infection. The carotid arteries were isolated. A TS420 perivascular flow module and nanoprobes (Transonic Systems Inc., Ithaca, NY, USA) were used to measure arterial blood flow. The signals were recorded constantly by a PowerLab 8/35 device (AD Instruments Ltd., Dunedin, New Zealand). The cavity between the artery and probe was filled with transparent gel, and injury was induced to external surface of the artery by attaching 1 × 2 mm filter paper soaked with 60% FeCl3 solution for 5 min. The time to occlusion (TTO), defined as the time at which no blood flow had been detected for > 5 min, was determined. The recording time was limited to 60 min.

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