CK and CK-MB measurement: Analysis of plasma CK and CK-MB enzymes were measured using the commercial kit (11447378 122, Roche) in Cobas c701 system (Roche Molecular Diagnostics, Pleasanton, CA, USA).

BUN measurement: Quantitative determination of serum urea level was done by using the commercial kit (Cat. No. 10171778 122, Roche) in Cobas 8000 autoanalyzer by spectrophotometric method.

TP I assay: Troponin I (Troponin I kit - DRG Diagnostic) levels were measured in the ELISA device (ELISA reader® -DAS) using a commercial kit (EIA-2952).

ALT and AST analysis: AST and ALT values were determined in cobas integra kit (ALT, 20764957322), (AST, 20764949322) as enzymatic.

LDH analysis: Serum LDH enzyme activity measurement was determined using the Cobas Integra kit (LDH, 03004732122).

Biochemical analysis of the muscle tissue: Homogenates were prepared from tissues for biochemical analysis on liver tissues. tGSH and MDA levels in the supernatants obtained from these homogenates were determined using appropriate methods in the literature.

Preparation of samples: At this stage of the study was weighed 0.2 g of each tissue sample removed. For MDA measurement, 1.15% potassium chloride solution was completed to 2 ml in phosphate buffer pH = 7.5 for tGSH measurement and homogenized in ice medium. The tubes were then centrifuged at +4°C at 10,000 rpm for 15 min. Supernatants were used as the analysis sample.

MDA measurement: MDA measurement is based on the spectrophotometric measurement (at 532 nm) of absorbance of a pink colored complex formed by thiobarbituric acid and MDA at high temperature (95°C) [23].

tGSH measurement: DTNB (5,5’-Dithiobis [2-nitrobenzoic acid]) in the measurement medium is a disulfide chromogen and is readily reduced by compounds with sulfhydryl groups. The resulting yellow color was measured spectrophotometrically at 412 nm [24].

Histopathological examination: All of the tissue samples were first identified in a 10% formaldehyde solution for light microscope assessment. Following the identification process, tissue samples were washed under tap water in cassettes for 24 h. Samples were then treated with a conventional grade of alcohol (70%, 80%, 90%, and 100%) to remove the water within tissues. Tissues were then passed through xylol and embedded in paraffin. Four-to-five micron sections were cut from the paraffin blocks and hematoxylin-eosin staining was administered. Their photos were taken following the Olympus DP2-SAL firmware program (Olympus Inc., Tokyo, Japan) assessment. Histopathological assessment was carried out by the pathologist blind for the study groups. The severity of histopathological findings in each section was scored between grade 0–3 (0-normal, 1-mild damage, 2-moderate damage, and 3-severe damage).

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