B16F10 cells were seeded in 6-well plate at a density of 5 × 104 cells/well and incubated for 16 h. They were then treated with various concentrations of peptide mixture in 2% FBS containing media for 3 days. To induce melanin production, 100 ng/ml α-MSH was co-treated and 200 μM arbutin was used as a positive control. Each well was treated with 200 μl of lysis buffer (Sigma-Aldrich) to collect the lysate. After quantitation of the protein using BCA kit (Thermo Fisher Scientific), 90 μg of protein from each group was incubated with 20 μl of 10 mM L-DOPA in 96-well plate for 30 min at 37°C. Absorbance for mixture at 475 nm was measured with a spectrophotometer.

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