MSCs, Calu‐3, and VERO E6 were infected with 100 μL (100 TCID50/mL) of a previously titrated SARS‐CoV‐2 wild strain, isolated from an infected patient. The virus was incubated for 1 hour and then removed; the medium was changed every 3 days. Cells were scored every other day and for 1 week using a light microscope to detect the appearance of cell rounding, detachment, degeneration, and/or syncytium formation, called hereafter cytopathic effect (CPE). To verify if the cells tested can be infected and allow SARS‐CoV‐2 replication even in the absence of CPE, at day 7 from infection, supernatants from each MSC, Calu‐3, and VERO E6 culture were collected and inoculated into VERO E6. CPE occurrence was monitored for 1 week.

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