A total of 207 non-duplicate M. catarrhalis isolates were collected from different inpatients at Shenzhen Nanshan People’s Hospital Shenzhen University, China, between January 1st, 2012 and December 31st, 2017 from the following sources: 161 from sputum (77.8%), 43 from throat swabs (20.8%), two (0.9%) from blood, and one (0.5%) from bronchoalveolar lavage fluid. The isolates were identified using the Phoenix 100 automated microbiology system (Becton Dickinson [BD], Franklin Lakes, NJ, USA), and their identities were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (IVD MALDI Biotyper; Bruker, Bremen, Germany). Staphylococcus aureus ATCC 29213 was used as the quality control strain.

All procedures involving human patients in this retrospective study were approved by the ethics committee of Shenzhen Nanshan People’s Hospital, according to the ethical standards of Shenzhen University and the 1964 Helsinki declaration and its later amendments, or comparable ethical standards (approval number: SZNS2017015). For this type of study, formal consent was not required.

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