We measured the hemolysis index semi-quantitatively using the Beckman Coulter LIH reagent in the AU5800 instrument. According to the manufacturer’s instructions, the measured hemolysis index was classified as N, +, ++, and +++, which represent approximately < 0.5, 0.5–0.99, 1–1.99, and 2–2.99 g/L of hemoglobin (a higher degree of hemolysis was not observed in the study period). To estimate hemolytic samples, the ratio of a positive hemolysis index (+, ++, and +++) to the total requested samples was compared before and after introduction of the Barricor tube. Outpatient results with a positive hemolysis index were usually reported with a warning comment. To ensure comparability, only tubes requested for stat tests in the third-floor phlebotomy unit in the two months before and after introduction of the Barricor tube were analyzed.

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