In all ensuing experiments, MS09:Chol and MS09:DOPE lipoplexes (MS09:siRNA w/ w = 16:1) contained 12 nM siRNA. MCF-7 and HT-29 cells were seeded in 6-well plates at densities of 3.0 ×10 5 cells/well and incubated as previously described. Cells were transfected with lipoplexes as previously described. Lipoplexes were assembled with 12 nM of either non-targeting siRNA or ON TARGETplus SMARTpool Human myc-siRNA (anti-c- myc-siRNA). Transfections were carried out in triplicate, followed by cell harvesting for total RNA and protein.

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