Raw data was acquired using MassLynx v 4.1 in multiple reaction monitoring mode. Raw files were processed using Skyline v 19. Protein-Peptide sequences were obtained from www.uniprot.org and settings optimised using custom synthesised peptides (Genscript USA). Peak intensity data were normalised to a spiked internal standard protein yeast enolase (Sigma, UK). Normalised data were exported to Microsoft Excel and analysed using SIMCA v 15 (Umetrics, Sweden) for multivariate analysis and Graphpad prism v 6 was used for statistical analysis.

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