The AH109 yeast cells were co-transformed with the following combinations of plasmids: pGADT7+pGBKT7, pGADT7+pGBKT7-FOXM1, pGADT7+pGBKT7-FOXM1△PLDLI, pGADT7-CtBP1+pGBKT7, pGADT7-CtBP1+pGBKT7-FOXM1, and pGADT7-CtBP1+pGBKT7-FOXM1△PLDLI. The transformed cells were selected in synthetic complete medium lacking Trp and Leu (SC-TL). The positive colonies were further grown in synthetic complete medium lacking His, Trp, and Leu (SC-HTL) to determine protein interactions. The β-galactosidase activity was measured following a protocol described previously 23.

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