A 1500-bp length of MDR1 promoter was cloned into pGL4.26 luciferase vector using KpnI and XhoI sites. The generated pGL4.26-pMDR1WT was used as a template to create its mutant vector in which the FOXM1 binding site GTAAACAA was mutated to GGTTTATT. The coding sequence of CtBP1 was cloned into pGADT7 empty vector using EcoRI and BamHI sites. Full-length coding sequences of FOXM1 and its mutant (FOXM1△PLDLI) were cloned into pGBKT7 empty vector using EcoRI and BamHI sites. Full-length coding sequences of FOXM1 and FOXM1△PLDLI were cloned into pCDNA3-MYC empty vector using HindIII and EcoRI sites. Full-length coding sequence of CtBP1 was cloned into pCDNA3-2×Flag empty vector using HindIII and EcoRI sites. All primers used for vector constructions were listed in Supplementary Table 1.

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