At 72 h post-transfection, siRNA-transfected cells were harvested and stained with fluorescein isothiocyanate-conjugated annexin V and phosphatidylinositol using an Annexin V Kit (Beckman Coulter, Brea, CA). A Becton Dickinson Accuri™ C6 flow cytometer was used to analyze the proportion of apoptotic cells.

To assess the chemoresistance of GC cell lines to CDDP and paclitaxel (PTX), NUGC4 (wild-type TP53) and MKN7 (mutant TP53) that had been transfected with siRNA-TRIM37 and its control were plated onto 6-well plates (NUGC4, 3 × 104 cells; MKN7, 5 × 104 cells per well) and incubated overnight under normal culture conditions. The cells were then incubated with CDDP (8 μM) and PTX (0.1 μM). At 48 h after the addition of the anticancer drug, apoptotic cell analysis was performed as mentioned above.

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