Total RNA was extracted from cell lines using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The reverse transcription reaction was performed using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). The total RNA of normal organs was purchased from Takara Bio Inc., Shiga, Japan (Human Total RNA Master Panel II (Cat. No. 636643). The abundance of mRNA was measured by qPCR using a StepOnePlus PCR system (Applied Biosystems), and cycle threshold (Ct) values were calculated with StepOne Software v2.0 (Applied Biosystems), using TaqMan Gene Expression Assays (Hs01116337_m1 for TRIM37; Applied Biosystems) according to the manufacturer's instructions. The results of gene expression were calculated as a ratio between TRIM37 and an internal reference gene (Hs01060665_g1 for β-actin; Applied Biosystems) that provided a normalization factor for the amount of RNA isolated from a specimen. This assay was performed in triplicate for each sample.

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