From the Cancer Genome Atlas (TCGA) database (https://cancergenome.nih.gov/), a total of 497 primary prostate cancer patients with gene expression profiles were identified and used in this study. Clinical characteristics at time of diagnosis, including PSA levels, Gleason score, and clinical stage were described. Disease-free survival (DFS) was the primary end point of this study. DFS was defined as the time from diagnosis to the first event, including biochemical recurrence, death, on the last follow-up. Between-group comparisons of DFS were performed by the Kaplan-Meier method and the log-rank test. Gene differential expression of TCGA pan-cancer was performed by TIMER DiffExp module 23.

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