For cell cycle distribution, the transfected cells were harvested and washed twice with cold PBS. Then cells were resuspended and fixed in 70% pre-cooled ethanol at 4 °C overnight. On the next day, cells were washed by PBS and treated with PI for 30 min away from light at room temperature. We used flow cytometry (FACScan; BD Biosciences, Shanghai, China) to examine the percentages of cells in G0/G1, S and G2/M phase.

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