After transfection with si-NC or si-DLEU2, approximately 1 × 106 HT29 cells were collected for flow cytometry assays, and DNA content was detected using propidium iodide (Sigma, San Antonio, USA) staining according to the methods described in a previous study 30. Analysis of cell cycle distribution used cell ModFit software (Beckman Coulter, South Kraemer, USA). Each experiment was repeated three times independently.

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