The cell viability was performed using the WST-1 Assay Kit (Beyotime Biotechnology, Shanghai, China) as previously described 24. Briefly, CRC cells transfected with siRNA were seeded in 96-well plates at the density of 2 × 103. After incubating for 24, 48, 72, 96 hours, 10 μl WST-1 reagent (Roche) was added into the relevant cells. SpctraMax M5 (Molecular Devices, San Francisco, CA, USA) was utilized to measure the cell proliferation rates.

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