Nude mice with intracranial xenografts were euthanized then the xenografts were removed and embedded in paraffin. The sections were cut 5 μm thick and mounted on poly-L-lysine coated glass slides. Slides were incubated at 80°C for 1 h and deparaffinized with xylene, hydrated with gradient ethanol and stained with hematoxylin and eosin staining (H&E) (Sigma-Aldrich, USA). Slides were meanwhile stained immunohistochemically with primary antibody of GFAP, Nestin, S-100, Oligo-2, Ki-67 and Vimentin. DAB was used to stain the slides and hematoxylin was used to counterstain the nucleus. The slides were analyzed by optical microscopy.

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