The tissue from the patient was fixed in 10% formalin, dehydrated with automatic tissue hydroextractor, then embedded in paraffin. Serial 5 μm thick slides were cut with a slicer, incubated for 1h at 80°C, deparaffinized with xylene for two times, hydrated with ethanol gradient and stained with hematoxylin and eosin (H&E) (Sigma-Aldrich, USA). In the meanwhile, immunohistochemical staining was performed for GFAP, Nestin, Oligo-2, S-100, Vimentin and Ki-67 detection. Endogenous peroxidase inactivation was conducted with 3% H2O2, and antigen retrieval was performed in a microwave. Afterward, primary antibodies were added to each slide and the sections incubated with biotin-labeled secondary antibodies for 10 min. The staining were carried out using the 3, 3'-diaminobenzidine substrate (DAB) (RD, America), counterstained with hematoxylin, then analyzed by optical microscopy.

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