Human Hep G2 cells were obtained from American Type Culture Collection and maintained in minimal essential medium supplemented with 10% fetal bovine serum, 1% glutamine, and 1% nonessential amino acids. These cells were cultured in 37 °C incubator under a humidified atmosphere with 5% CO2. For study, Hep G2 cells were treated with 50 mM ethanol or 175 μM ACE for 24 h.

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