Human Hep G2 cells were obtained from American Type Culture Collection and maintained in minimal essential medium supplemented with 10% fetal bovine serum, 1% glutamine, and 1% nonessential amino acids. These cells were cultured in 37 °C incubator under a humidified atmosphere with 5% CO2. For study, Hep G2 cells were treated with 50 mM ethanol or 175 μM ACE for 24 h.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.