The serum ACE was measured by Sigma-Aldrich Colorimetric ACE assay kit. Briefly, 25 μL serum was mixed with 75 μL dilution buffer and incubated at room temperature for 1 h in dark. The absorption was determined at 405 nm. The concentration of ACE was estimated following the protocols of the manufacturer.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.