Cell apoptosis analysis was performed by flow cytometry using a fluorescein isothiocyanate (FITC) and propidium iodide (PI) kit (Vazyme, Nanjing, China). Cells were harvested after treatment with miR-125b mimic or inhibitor and washed with precooled PBS. The cells were mixed with 500 μL of binding buffer and 5 μL of FITC and PI and incubated for 10 minutes at room temperature (20 to 25 °C). Apoptosis rates were then measured by flow cytometry (FACS, Partec AG, Arlesheim, Switzerland). The percentage of apoptotic cells was calculated. Each experiment contained three replicates for each condition.

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