Total RNA was extracted from the tissues and cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. For miR-125b detection, Taqman assays were employed, and U6 was used as an internal control. For mRNA analysis, total RNAs were reverse transcribed into cDNA using the RT MasterMix Kit (Abm). Then, qRT-PCR was performed using SYBR Green-I as the fluorogenic dye. The relative expression fold changes were calculated by the 2-∆∆CT method.

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