Construction of recombinant lentiviral vectors and transfection

Recombinant lentivirus-V5-D-TOPO-ID1-EGFP (LV-ID1) and control vector lentivirus-V5‐D-TOPO-EGFP (LV-control) were constructed. At 30-50% confluence, typically 24 h after plating, B-ALL cells were transfected with LV-ID1 and LV-control using 5 µg/ml polybrene (Genechem, Shanghai, China). After 5 days of infection, B-ALL cells expressing green fluorescent protein (GFP) protein was evaluated using fluorescence microscopy and flow cytometry, and the cells stably overexpressing ID1 were confirmed by real-time PCR and western blot.

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