Small interfering RNA (siRNA)-mediated gene silencing

B-ALL cells were seeded at a density of 1×105 cells/well into a 6-well plate and cultured before transfection. After 12 hours, CCRF-SB and Sup-B15 cells were transfected with synthesized siRNA specifically targeting human USP1 (USP1-siRNA) (TransheepBio, Shanghai, China) or human ID1 (ID1-siRNA) (Santa Cruz) using Lipo6000 Transfection Reagent (Beyotime) according to the manufacturer's procedures. Meanwhile, a scrambled siRNA served as a negative control (NC-siRNA) (TransheepBio). After 48 hours of transfection, the RNA and protein were extracted and analyzed respectively.

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