After treatment, B-ALL cells were harvested and centrifuged, then fixed with 4% paraformaldehyde for 15 min and washed with PBS 3 times. Cell membrane permeabilization was performed with 0.1% Triton-X 100 (Beyotime) for 30 min. Afterwards, cells were incubated with fresh goat serum (5%) in following 1 h, cells were probed with specific primary antibody against ID1 at 4 °C overnight. After washing with PBS 3 times again, cells were incubated with the corresponding fluorescent-labeled secondary antibody (Beyotime). Finally, DAPI (Beyotime) was used to stain the nuclei. Fluorescence images were captured under a fluorescence microscope (Leica DM4000B, Wetzlar, Germany).

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