Cancer cells were seeded into each well of 96-well plates a day prior to treatment. After treatment with alvespimycin, cytochalasin B, or trichostatin A, 180 μL of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution was added to each well and incubated for 4 h. The absorbance of the solution was quantified by measuring at a 595-nm wavelength with a spectrophotometer.

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