To describe the effect of the combination treatments with Dasatinib/Rapamycin or Dasatinib/Torin-2 we calculate the Combination Index (CI) applying the Chou-Talalay-Method 34 as followed:

The calculations were done with the software CalcuSyn. We used normalized viability values determined by MTT assays as described above. For the calculation the viability values were converted to the “Fraction Affected” (FA) 35 as followed:

For the calculation, a constant dilution factor for both drugs is necessary. To test different concentration ranges we used various dilution factors (such as 1.5, 2.0 or 2.5). Three replicates were performed for each experiment. For calculation of the CI we used the results of at least two independent experiments.

The calculation of the Dose Reduction Factor (DRF) was done as followed:

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