Cell viability was assessed by MTT assay. B16/F10 (5 × 103) and MDCK (1 × 104) cells were grown in 96-well plates and treated with JIB extract (0-100 μg/ml), cisplatin (0-10 μg/ml), and 5-FU (0-2 μg/ml) for 24, 48, and 72 hrs, respectively. After incubating the cells with these drugs, the medium was discarded and replaced with MTT solution (500 μg/ml) and incubated for 8 hrs. After removing the MTT solution, the crystals were dissolved in DMSO, and the absorbance was measured at 550 nm. The percentage of cell viability was calculated using the following formula: treating/untreated × 100%, and the data were presented as mean ± SD. The experiment was repeated three times under the same conditions.

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