For analysis of pl-CSA, a recombinant VAR2CSA labeled with a 6×His tag (rVAR2), specifically interaction with pl-CSA, was expressed by inserting this fragment into a pET28a (+) vector that was transformed into Escherichia coli BL21, and was purified using a Ni2+ affinity column (cat. no. DP101-01; Transgen Biotech Co., Ltd., Beijing, China) from the supernatant of bacterial culture. The purified rVAR2 was used in the following experiments.

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