The R software package, CIBERSORT, was adopted to calculate the abundance of 22 leukocyte subtypes in 502 tumor samples, and a threshold of P-value <0.05 was considered as cut-off criteria 23. Furthermore, Wilcoxon rank-sum test was performed to analyze the difference of immune cells infiltration in low- and high- TMB groups, with the R software package, vioplot was used to visualize the difference.

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